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Adaptive Optics for Biological Imaging
book

Adaptive Optics for Biological Imaging

by Joel A Kubby
April 2013
Intermediate to advanced content levelIntermediate to advanced
388 pages
16h 6m
English
CRC Press
Content preview from Adaptive Optics for Biological Imaging
56 Methods
Again the normalized optical coordinates ν and u as dened in Equations 3.8 and 3.9 are used.
From Equation 4.6, we see that the aberrations have a direct eect on the amplitude PSF of the imag-
ing lens. In Section 3.4, it is explained how the PSF relates to the nal image of the CFM.
To illustrate the eect of aberrations on the imaging quality of the CFM, we calculated the image
of an innitely small uorescent object using Equations 4.6 and 3.12 under the assumption that the
uorescence wavelength is equal to the excitation wavelength (i.e., β = 1). Figure 4.3 shows the result
for the unaberrated wavefront (le column) and the ab ...
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Publisher Resources

ISBN: 9781439850190