Again the normalized optical coordinates ν and u as dened in Equations 3.8 and 3.9 are used.
From Equation 4.6, we see that the aberrations have a direct eect on the amplitude PSF of the imag-
ing lens. In Section 3.4, it is explained how the PSF relates to the nal image of the CFM.
To illustrate the eect of aberrations on the imaging quality of the CFM, we calculated the image
of an innitely small uorescent object using Equations 4.6 and 3.12 under the assumption that the
uorescence wavelength is equal to the excitation wavelength (i.e., β= 1). Figure 4.3 shows the result
for the unaberrated wavefront (le column) and the ab ...
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