Chapter Eight

Scanning optical microscopy

Colin J.R. Shepparda    Department of Engineering Science, Oxford University, Oxford, United Kingdom, colinjrsheppard@gmail.coma Current addresses: School of Chemistry, University of Wollongong, Wollongong, NSW, Australia and Istituto Italiano di Tecnologia, Genova, Italy.

Abstract

Microscopy using a scanning approach is reviewed, with numerous references to previous work, as at 1987, before the confocal laser scanning microscope was widely available commercially. The advantages of microscope imaging using a scanning technique are described. The principles of confocal microscopy are introduced. Use of Bessel beam illumination in a confocal microscope is discussed. This combination can increase spatial resolution ...

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