All layers in Anime Studio are 2D. They do understand the concept of stacking, which essentially makes them 2.5D, but all content is drawn in 2D and placed on 2D layers. But, even though the content is 2D, Anime Studio supports cameras that can manipulate the project in 3D.
Imagine a billboard with a 2D drawing on it. If you have a remote-controlled helicopter, you could fly it around the billboard looking at it from all different directions. This is how the cameras work in Anime Studio.
The various Camera tools work in a similar manner to the Workspace tools, but they actually alter the main window view and can be animated. Figure 25.1 shows the Camera tools available in the Tools palette.