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Bacteriophage Tail Fibers as a Basis for Structured Assemblies

Book Description

This concise monograph series focuses on the implementation of various engineering principles in the conception, design, development, analysis and operation of biomedical, biotechnological and nanotechnology systems and applications. Authors are encouraged to submit their work in the following core topics, but authors should contact the commissioning editor before submitting a proposal: BIoMeDIcAL DeVIceS & MATeRIALS Trauma Analysis Vibration and Acoustics in Biomedical Applications Innovations in Processing, Characterization and Applications of Bioengineered Materials Viscoelasticity of Biological Tissues and Ultrasound Applications Dynamics, and Control in Biomechanical Systems Clinical Applications of Bioengineering Transport Phenomena In Biomedical Applications Computational Modeling and Device Design Safety and Risk Analysis of Biomedical Engineering Modeling and Processing of Bioinspired Materials and Biomaterials NANoMeDIcAL DeVIceS & MATeRIALS Bio Nano Materials Nano Medical Sciences Materials for Drug & Gene Delivery Nanotechnology for Central Nervous System Nanomaterials & Living Systems Interactions Biosensing, Diagnostics & Imaging Cancer Nanotechnology Micro & Nano Fluidics Environmental Health & Safety Soft Nanotechnology & Colloids

Table of Contents

  1. Cover
  2. TitlePage
  3. copyright
  4. Contents
  5. Series Editors’ Preface
  6. Dedication
  7. Foreword
  8. Acknowledgements
  9. Abstract
  10. 1. Introduction
  11. 2. Tail fiber function and structure
    1. 2.1 Hypothesis
    2. 2.2 Staged or ordered assembly
    3. 2.3 Possible applications
  12. 3. Tail fiber production and purification
  13. 4. Tail fiber modifications
    1. 4.1 Deletions and insertions
    2. 4.2 Coiled-coil assembly segment modifications
      1. 4.2.1 Biological role of the gp 37 coiled-coil
      2. 4.2.2 Coiled-coils as intrinsic chaperones
    3. 4.3 Inserts conferring novel functionality
      1. 4.3.1 Insertion and testing of an antibody binding epitope
      2. 4.3.2 Insertion and testing of a biotinylation site
      3. 4.3.3 Attaching magnetic nanoparticles via a biotin linkage
      4. 4.3.4 Potential tail fiber-nanoparticle system improvements
  14. 5. Conclusions
  15. Appendices
    1. A. Tail fiber purification
    2. B. Preparation of head-tail (HT) complexes
    3. C. Purified tail fiber assay
    4. D. Analytical ultracentrifugation
    5. E. Electron microscopy
    6. F. Bacteria and bacteriophage strains
    7. G. Media and buffers
    8. H. Phage stock growth and construction of phage with multiple mutations
    9. I. Transfer of engineered mutations into phage
    10. J. PCR and sequencing primers
    11. K. Adsorption rate measurements
    12. L. Construction of extended coiled-coil region
    13. M. Mab binding assays
    14. N. Construction of biotinylated phage
    15. O. Western blot and SDS-PAGE analysis
    16. P. Purification of biotinylated tail fibers
    17. Q. Preparation of nanoparticles
    18. R. Attachment of nanoparticles to biotinylated tail fibers
    19. S. Dynamic light scattering (DLS)
    20. T. AC magnetic susceptometry theory
    21. U. Magnetic susceptibility measurements
  16. About the Authors
  17. References
  18. Index
  19. Backcover