Multiplexed, lateral flow, polymerase chain reaction (PCR) techniques for biological identification

W.M. Nelson, G.W. Long and L.M. Cockrell,    Tetracore, Inc., USA


This chapter describes the history and development of the real-time polymerase chain reaction (PCR). It describes the types of real-time PCR chemistries and analyses the advantages of real-time PCR compared with other techniques. The chapter goes on to look at relevant considerations when designing a real-time PCR assay – such as contamination, controls and reverse transcription real-time PCR. Instrument platforms are covered, including portable real-time PCR detection, with examples of available systems.

Key words

real-time polymerase chain reaction; instrumentation platform; ...

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