Isothermal amplification of specific sequences

Y. Tong,    New England Biolabs, USA


This chapter focuses on the mechanisms of isothermal amplification of specific sequences. More than 15 different isothermal amplification technologies – including loop-mediated isothermal amplification (LAMP); strand displacement amplification (SDA); cross priming amplification (CPA); rolling circle amplification (RCA); helicase-dependent amplification (HDA); recombinase polymerase amplification (RPA); nucleic acid sequence-based amplification (NASBA) and transcription mediated amplification (TMA) – have been grouped for discussion and comparison. The following aspects are compared and presented for each platform: amplification scheme, primer design, ...

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