7

 

 

Automatic Single-Cell Transfer Module

 

Huseyin Uvet

Yildiz Technical University Istanbul, Turkey

Akiyuki Hasegawa

Tokyo Women's Medical University Tokyo, Japan

Kenichi Ohara, Tomohito Takubo, Yasushi Mae, and Tatsuo Arai

Osaka University Osaka, Japan

CONTENTS

7.1 Introduction

7.2 Materials and Methods

7.2.1 Cell Types and Preparation

7.2.2 Penicillin-Streptomycin and Bottom Surface Treatment

7.2.3 Cell Suction System

7.2.4 Manufacture of the Microfluidic Chip

7.2.5 Valve Control Principle

7.2.6 Vision Systems

7.2.7 Cell Detection/Tracking and Control

7.3 Experimental Results

7.3.1 Oocyte and Fibroblast Suction

7.3.2 Direction Control

7.4 Discussion and Conclusion

References

Abstract

Conventional hybrid microfluidic systems have many ...

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