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Figure 5.10 (A) Optical micrograph showing six microchemostats that operate in parallel
on a single chip. Various inputs have been loaded with food dyes to visualize channels
and sub-elements of the microchemostats. The coin is 18 mm in diameter. (B) Optical
micrograph showing a single microchemostat and its main components. Scale bar, 2 mm.
(C) Schematic diagram of a microchemostat in continuous circulation mode. Elements such
as the growth loop with individually addressable connected segments, the peristaltic pump,
supply channels, and input/output ports are labeled. (D) Isolation of a segment from the
rest of the growth chamber during cleaning and dilution mode. A lysis buffer (indicated in
red) is introduced into the chip through the lysis buffer port. Integrated microvalves direct
the buffer through the segment, ﬂushing out cells, including those adhering to chamber
walls. The segment is then rinsed with fresh sterile medium and reunited with the rest of
the growth chamber.
Figure 5.12 Schematic of the fabrication of agarose microﬂuidic devices with (right) and
without (left) embedded cells.
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