167In Vivo Biodetection Using Surface-Enhanced Raman Spectroscopy
toluidine blue (TB), a potential antitumor drug for photodynamic therapy of tumor, with
calf thymus DNA (ctDNA). The SERS experiment was based on the Raman spectroscopy
analysis of solutions prepared with TB, ctDNA, and silver colloid. Based on the intensity of
specic peaks of the spectra, it was proposed that TB could partially intercalate into DNA
with ring C
1
NC
1
buried into DNA base pairs, while the steric hindrance resulting from the
substituents blocked the insertion from the C
6
NC
6
side of TB.
7.3.2.4 Monitoring of Conformational Changes Induced by Target Molecules
Aptamers are single-stranded DNA/RNA oligomers that fold into 3D conformations in the
presence of specic mo ...