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Biosensors Based on Nanomaterials and Nanodevices
book

Biosensors Based on Nanomaterials and Nanodevices

by Jun Li, Nianqiang Wu
December 2017
Intermediate to advanced content levelIntermediate to advanced
517 pages
21h 5m
English
CRC Press
Content preview from Biosensors Based on Nanomaterials and Nanodevices
337Graphene-Based Electrochemical Biosensors
Au NPs and subsequently horseradish peroxidase (HRP)–anti-α-fetoprotein. The remain-
ing electrode surface was passivated with bovine serum albumin. In the presence of H
2
O
2
but not the antigen α-fetoprotein, HRP catalyzed the reduction of H
2
O
2
and a cathodic cur-
rent peak corresponding to the regeneration of PTH at the electrode was produced. When
α-fetoprotein was present along with H
2
O
2
, the binding of α-fetoprotein to the HRP–anti-
α-fetoprotein caused a decrease in cathodic current, possibly because the active site of the
HRP was blocked or H
2
O
2
could no longer reach the enzyme.
For graphene-based label-free electrochemical immunosensors no matter the use of
binding-induced changes in current respon ...
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Publisher Resources

ISBN: 9781466551527