
438 Biosensors Based on Nanomaterials and Nanodevices
that are not transparent. The probe ssDNA-modied nanoparticles and capture ssDNA-
modied aluminum surface are added in the milk for hybridization. After hybridizing
for 3 h, the aluminum surface is taken out, washed with phosphate buffer and tested by
DSC. The pH values and ion concentrations of milk are adjusted to 10 mM Tris-HCl at
pH 7.5, 100 mM NaCl, and 1 mM EDTA. Figure 17.4a shows the DSC curves of lead–tin
nanoparticles collected at different concentrations of target ssDNA, where the melting
peaks are attributed to immobilized lead–tin nanoparticles. The method has been used
to detect ...