book

Cellular Signal Transduction in Toxicology and Pharmacology

by Jonathan W. Boyd, Richard R. Neubig

April 2019

Intermediate to advanced

336 pages

10h 30m

English

Wiley

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1.1 Starting Big, but Ending Small1.2 Responding to Our Environment: Sensory Perception Begins and Ends with Signal Transduction1.3 Primary Transport Systems Involved in Signal Transduction1.4 Key Organelles Involved in Signal TransductionReferences
2.1 Posttranslational Modifications and Their Roles in Signal Transduction2.2 Receptors2.3 Receptor Signaling Mechanisms2.4 Receptor Tyrosine Kinases2.5 Steroid Receptors2.6 Reactive Oxygen Species (ROS)2.7 SummaryReferences
3.1 Introduction3.2 Cellular Information Flow: Mechanisms of Cellular Signal Integration and Regulation3.3 Crosstalk and Functional Signal Integration in Response to Insulin in Hepatocytes3.4 Systemic Signal Integration3.5 Dysregulation of Insulin Signaling in the Etiology of Type 2 DiabetesReferences
4.1 Introduction4.2 Fibrosis as an Example of Complex Signaling4.3 Cancer Drug Resistance: Complex Cellular and Population Changes4.4 SummaryReferences
5.1 Overview of Basic Experimental Design5.2 Aseptic Technique5.3 Biological Sample Collection, Processing, and Pretreatment Technology5.4 Sample Storage5.5 Common In Vitro Studies in Toxicology/Pharmacology5.6 Common In Vivo Studies in Toxicology5.7 Basic Advantages and Disadvantages Associated with Sample Types5.8 Human Epidemiology Studies5.9 Examples of Tox‐ and Pharm‐Based Experiments Relevant to Signal Transduction Endpoints5.10 Coupling Experimental Results Within the Larger Literature Framework to Generate InformationReferences
6.1 Introduction6.2 High‐Throughput Versus High‐Content Data6.3 Methods to Measure Signal Transduction Data6.4 Techniques to Generate Large Datasets for Signal Transduction Network Analysis6.5 Bioenergetics6.6 Relating Signaling to Cellular Outcome Using Relevant Assays6.7 SummaryReferencesSuggested Reading

7.1 Introduction7.2 Network Construction7.3 Facing the Network Analysis7.4 Employing Centrality Analysis to Evaluate Stressed Biological Systems7.5 Interference Notion: How to Perform Virtual Knockout Experiments on Biological Networks7.6 Network Analysis Software7.7 ConclusionsReferences
8.1 Introduction8.2 Classification of Toxic Agent and Exposure Effects: A Toxicological Perspective8.3 Early Cellular Changes Post‐exposure8.4 Experimentally Testing Early Cellular Changes that May Contribute to Exposure Sensing and ResponseReferencesAppendix A
9.1 Translational Research and a Spatiotemporal Understanding of Signal Transduction9.2 Integrating Second Messengers into Signal Transduction9.3 Understanding Crosstalk in Signal Transduction9.4 Posttranslational Modifications (PTMs) and Target Identification in Signal Transduction9.5 Epigenetic Endpoints in Signal Transduction9.6 The Integration of Nutrition and Signal TransductionReferences

Overview

Covering a key topic due to growing research into the role of signaling mechanisms in toxicology, this book focuses on practical approaches for informatics, big data, and complex data sets.

Combines fundamentals / basics with experimental applications that can help those involved in preclinical drug studies and translational research
Includes detailed presentations of study methodology and data collection, analysis, and interpretation
Discusses tools like experimental design, sample handling, analytical measurement techniques