Instruments
2.1 Introduction
The basic requirements for a confocal scanning optical microscope (CSOM) are point illumination, point detection, a confocal lens system, and a method of scanning the image. A number of different optical systems have been used to fulfill these requirements. They range in complexity from simple microscopes, using a laser, pinhole, and a scanning stage, to more complicated instruments employing white light sources, galvanometer mirrors, acousto-optic cells, or Nipkow disks for scanning. In this chapter we will discuss a few variations of the CSOM. The different approaches will be compared, pointing out the most common applications for each instrument.
The chapter begins with Section 2.2, The Confocal Scanning ...
Get Confocal Scanning Optical Microscopy and Related Imaging Systems now with the O’Reilly learning platform.
O’Reilly members experience books, live events, courses curated by job role, and more from O’Reilly and nearly 200 top publishers.
