O'Reilly logo

Emerging Health Trends: A Look at Where Science Will Take Us (Collection) by Karl S. Drlica, David S. Perlin, Paul J. H. Schoemaker, Joyce A. Schoemaker, Greg Gibson, Anthony DePalma

Stay ahead with the world's most comprehensive technology and business learning platform.

With Safari, you learn the way you learn best. Get unlimited access to videos, live online training, learning paths, books, tutorials, and more.

Start Free Trial

No credit card required

B. Cloning genes

We describe a typical cloning procedure, starting with an illustration of how restriction enzymes work in the process.

Restriction enzymes

One of the first restriction enzymes, dubbed EcoR1, was discovered in E. coli and found to cut only within the nucleotide sequence GAATTC (or its mirror image CTTAAG), and specifically between the G and A. The enzyme cuts both DNA strands of the double helix creating small single-stranded fragments at the cut sites that stick out:

Double-stranded DNA before cutting:

XXXXXXXGAATTCXXXXXXX

XXXXXXXCTTAAGXXXXXXX

Double-stranded DNA after cutting with EcoR1:

XXXXXXXG     AATTCXXXXXXX

XXXXXXXCTTAA GXXXXXXX

Note that the Xs in this example can be A, T, C, or G, corresponding to the basic alphabet ...

With Safari, you learn the way you learn best. Get unlimited access to videos, live online training, learning paths, books, interactive tutorials, and more.

Start Free Trial

No credit card required