ACTIVITY OF LIPOSOMAL
ANTIMICROBIC PREPARATIONS
CONCERNING STAPHYLOCOCCUS
AUREUS
N. N. IVANOVA, G. I. MAVROV, S. A. DERKACH, and E. V. KOTSAR
CHAPTER 7
CONTENTS
7.1 Introduction .....................................................................................................134
7.2 Materials and Methods ....................................................................................134
7.2.1 The Receiving of Liposomes .............................................................134
7.3 Discussion and Results ...................................................................................135
7.4 Conclusion ......................................................................................................136
Keywords .................................................................................................................137
References ................................................................................................................137
134 Engineering of Polymers and Chemical Complexity
7.1 INTRODUCTION
It has been found the antimicrobic activity of liposomal lincomycin depends on the
composition and charge of liposomes. The antimicrobic activity of liposomal linco-
mycin, obtained on the basis of egg lecithin is higher than antimicrobic activity of
the solution lincomycin in three times concerning planktonic cells of Staphylococ-
cus aureus. Negatively charged liposomes received on the basis of polar lipids and
lincomycins were more effective, than neutral liposomes. The minimum inhibitory
concentration (MIC) of them is less of MIC of lincomycin in seven times concerning
planktonic cells of Staphylococcus aureus. The antimicrobic activity of liposomal ben-
zoyl peroxide (BP) obtained on the basis of egg lecithin was in 14 times higher than
antimicrobic activity of the solution BP concerning of Staphylococcus aureus.
The skin of patients with atopic dermatitis planted on different microorgan-
isms, whose number is much bigger than the skin of healthy people. For example,
Staphylococcus aureus sow from the skin of patients with atopic dermatitis in 80–100%
of cases. Skin diseases of microbial etiology in most cases basic drug treatment and
prevention are antibiotics. Widespread use of antibiotics has negative consequences,
one of which is the emergence of pathogens with resistance to penicillin, gentamicin,
tetracycline, methicillin, lincomycin, and sulfonamides, as well as a new generation of
antibiotics––quinolones and cephalosporins. Consequently, the problem of prevention
and treatment of infectious diseases is urgent. One of the ways of its solution is the
introduction of new chemotherapeutic agents into medical practice.
      -

changing the nature of their actions. Thus, in the work [4] it was shown that, despite

Staphylococcus aureus was higher than the intact benzyl pen-
         
agents in liposomal form relatively to Staphylococcus aureus.
7.2 MATERIALS AND METHODS
The strain ATCC 25923 Staphylococcos aureus was taken from SE “ Mechnicov In-
stitute of Microbiology and Immunology AMSU”. We have also used the following
items: egg lecithin (Ukraine, “Biolek”), DMSO (Russia), the mixture of negatively
charged lipids that were obtained by the original technology of Dr. Nina Ivanova, sub-


7.2.1 THE RECEIVING OF LIPOSOMES
The substance of BP dissolved in chloroform due to its poor solubility in aqueous
solutions and added to an alcohol or chloroform solution of lipids in the ratio of BP––
Lipids 1:10 and 1:20. The liposomes were obtained by evaporating the lipids and anti-
biotics on a rotary vacuum evaporator (Switzerland). Next mixture was suspended in
sterile buffered saline. Liposomes prepared in the extruder EmulsiFlex–C5 (Canada

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