
262 Engineering of Polymers and Chemical Complexity
mixture was kept at 4ºC for 16 hr to complete the immobilization process. The mecha-
nism of the activation process and enzyme immobilization is presented in Scheme 1.
SCHEME 1 Mechanism of activation and immobilization process.
DETERMINATION OF IMMOBILIZED ENZYME ACTIVITY
The catalytic beads were mixed with 0.1 M Lactose-Tris-HCl buffer solution of (pH
4.8 with stirring, 250 rpm, at room temperature for 30 min. Samples were taken every
5 min to assess the glucose production using glucose kit. Beads activity is given by the
angular coefficient of the linear plot of the glucose production as a ...