Genome Engineering for Crop Improvement

Book description

In recent years, significant advancements have been made in the management of nutritional deficiency using genome engineering—enriching the nutritional properties of agricultural and horticultural crop plants such as wheat, rice, potatoes, grapes, and bananas. To meet the demands of the rapidly growing world population, researchers are developing a range of new genome engineering tools and strategies, from increasing the nutraceuticals in cereals and fruits, to decreasing the anti-nutrients in crop plants to improve the bioavailability of minerals and vitamins.

 

Genome Engineering for Crop Improvement provides an up-to-date view of the use of genome editing for crop bio-fortification, improved bioavailability of minerals and nutrients, and enhanced hypo-allergenicity and hypo-immunogenicity. This volume examines a diversity of important topics including mineral and nutrient localization, metabolic engineering of carotenoids and flavonoids, genome engineering of zero calorie potatoes and allergen-free grains, engineering for stress resistance in crop plants, and more. Helping readers deepen their knowledge of the application of genome engineering in crop improvement, this book:

 

  • Presents genetic engineering methods for developing edible oil crops, mineral translocation in grains, increased flavonoids in tomatoes, and cereals with enriched iron bioavailability
  • Describes current genome engineering methods and the distribution of nutritional and mineral composition in important crop plants
  • Offers perspectives on emerging technologies and the future of genome engineering in agriculture

 

Genome Engineering for Crop Improvement is an essential resource for academics, scientists, researchers, agriculturalists, and students of plant molecular biology, system biology, plant biotechnology, and functional genomics.

Table of contents

  1. Cover
  2. Title Page
  3. Copyright Page
  4. Dedication Page
  5. List of Contributors
  6. Preface
  7. About the Editor
  8. Acknowledgments
  9. 1 An Overview of Genome‐Engineering Methods
    1. 1.1 Introduction
    2. 1.2 ZFNs
    3. 1.3 TALENs
    4. 1.4 CRISPR‐Cas System
    5. 1.5 CRISPR‐Cpf1
    6. 1.6 Conclusions
    7. Acknowledgements
    8. References
  10. 2 Distribution of Nutritional and Mineral Components in Important Crop Plants
    1. 2.1 Introduction
    2. 2.2 Exploring Nutrient Distribution in Grain
    3. 2.3 Exploring the Mineral Distribution in Grain
    4. 2.4 Prospect
    5. Acknowledgement
    6. References
  11. 3 Application of Genome Engineering Methods for Quality Improvement in Important Crops
    1. 3.1 Introduction
    2. 3.2 Evolution and Historical Perspective of Genome Engineering
    3. 3.3 CRISPR/Cas Genome Editing Systems
    4. 3.4 Application of CRISPR/Cas System for Crops Quality Improvement
    5. 3.5 Regulatory Measures for Genome Engineering Crops
    6. 3.6 Conclusion
    7. Acknowledgement
    8. References
  12. 4 Genome Engineering for Enriching Fe and Zn in Rice Grain and Increasing Micronutrient Bioavailability
    1. 4.1 Introduction
    2. 4.2 Genes Related to Uptake of Fe and Zn from the Soil
    3. 4.3 Fe and Zn Biofortification using the SDN‐1 Approach
    4. 4.4 Fe and Zn Biofortification Using the SDN‐2 Approach
    5. 4.5 Fe and Zn Biofortification Using the SDN‐3 Approach
    6. 4.6 Future Thrust and Implications of SDN‐1, ‐2, and ‐3
    7. References
  13. 5 Development of Carotenoids Rich Grains by Genome Engineering
    1. 5.1 Introduction
    2. 5.2 Nutritional Quality Improvement Through Pathway Engineering
    3. 5.3 Crop Improvement through Genetic Engineering Techniques
    4. 5.4 Improvement of Carotenoid in Grain Crops through CRISPR/Cas9
    5. 5.5 Improvement of Carotenoid in Grain Crops Through RNAi
    6. 5.6 Future Perspectives and Conclusion
    7. References
  14. 6 CRISPR‐Cas9 System for Agriculture Crop Improvement
    1. 6.1 Introduction
    2. 6.2 Genome Engineering
    3. 6.3 Tools for Genome Engineering
    4. 6.4 CRISPR/Cas Beyond Genome Editing
    5. 6.5 CRISPR/Cas and Crop Improvement
    6. 6.6 Application of Genome Engineering Tools in Metabolic Engineering
    7. 6.7 Future Prospective
    8. References
  15. 7 Contribution of Crop Biofortification in Mitigating Vitamin Deficiency Globally
    1. 7.1 Introduction
    2. 7.2 Effect of Vitamins on Human Health and Their Sources
    3. 7.3 Plan Biofortification to Overcome Vitamin Deficiency
    4. 7.4 Conclusion
    5. Acknowledgments
    6. References
  16. 8 Genome Editing Approaches for Trait Improvement in the Hairy Root Cultures of the Economically Important Plants
    1. 8.1 Introduction
    2. 8.2 Secondary Metabolites and Hairy Root Culture: An Insight
    3. 8.3 Genome Editing Process in Plants
    4. 8.4 Plant Hairy Root Culture as a Model for Genome Engineering
    5. 8.5 Conclusions
    6. Acknowledgements
    7. References
  17. 9 Phytic Acid Reduction in Cereal Grains by Genome Engineering
    1. 9.1 Introduction
    2. 9.2 Genes Involved in Phytic Acid Biosynthesis
    3. 9.3 Potential Targets and Strategies to Achieve Low Phytate Wheat
    4. 9.4 Evolution of Genome Engineering for Trait Development in Wheat
    5. 9.5 Future Implications
    6. Acknowledgements
    7. References
  18. 10 Genome Engineering for Nutritional Improvement in Pulses
    1. 10.1 Introduction
    2. 10.2 Need for Nutritional Improvement in Pulses
    3. 10.3 Nutritional Defects in Pulses Targeted for Genetic Engineering
    4. 10.4 Genome Engineering as an Alternate to Conventional Breeding
    5. 10.5 Conclusive Discussion
    6. References
  19. 11 The Survey of Genetic Engineering Approaches for Oil/Fatty Acid Content Improvement in Oilseed Crops
    1. 11.1 Background
    2. 11.2 Soybean: Triumph Oil Crop
    3. 11.3 Camelina sativa: Biofuel and Future Ready Crop
    4. 11.4 Conclusion
    5. Acknowledgments
    6. References
  20. 12 Genome‐Editing Mediated Improvement of Biotic Tolerance in Crop Plants
    1. 12.1 Introduction
    2. 12.2 Plant Defense Response
    3. 12.3 Genome Engineering Tools for Engineering Disease Resistance
    4. References
  21. 13 Genome Engineering and Essential Mineral Enrichment of Crops
    1. 13.1 Introduction
    2. 13.2 Root Engineering of Cereals: A Promising Strategy to Improve Nutrient Efficiency, Biofortification, and Drought Tolerance
    3. 13.3 Use of Genome Edited Plants in Phytoremediation
    4. 13.4 Genetic Engineering and Crop Biofortification
    5. 13.5 Genome Engineering Technology and Its Use in Essential Mineral Enrichment of Crop Plants
    6. 13.6 Conclusion
    7. References
  22. 14 Genome Editing to Develop Disease Resistance in Crops
    1. 14.1 Introduction
    2. 14.2 Traditional Approaches to Develop Disease Resistance in Crops
    3. 14.3 Genome Editing‐a New Way Forward
    4. 14.4 Genome Editing Examples to Develop Disease Resistance in Crops
    5. 14.5 Recent Trends in Genome Editing
    6. 14.6 Conclusion and Prospects
    7. References
  23. 15 Biotechnological Approaches for Nutritional Improvement in Potato (Solanum tuberosum L.)
    1. 15.1 Introduction
    2. 15.2 Genetic Transformation of Potato
    3. 15.3 Protein Engineering of Potato for Protein Content
    4. 15.4 Genetic Engineering of Potato for Starch Modification
    5. 15.5 Lipids Biosynthesis Engineering in Potato
    6. 15.6 Vitamins Genetic Engineering in Potato
    7. 15.7 Metabolic Engineering of Potato for Enhanced Mineral Content
    8. 15.8 Pathway Engineering for the Functional Secondary Metabolites
    9. 15.9 Future Prospective and Conclusions
    10. References
  24. 16 Genome Engineering Strategies for Quality Improvement in Tomato
    1. 16.1 Introduction
    2. 16.2 Genome Editing Systems in Plants
    3. 16.3 Current Applications of Genome Editing in Tomato Improvement
    4. 16.4 Challenges and Future of Genome Editing in Tomato
    5. References
  25. 17 Genome Editing for Biofortification of Rice
    1. 17.1 Introduction
    2. 17.2 Genome Editing and its Tools
    3. 17.3 Genome Editing for Biofortification of Rice
    4. 17.4 Genome Editing for Improvement of Agronomic Traits in Rice
    5. 17.5 Conclusion and Future Aspects
    6. Acknowledgment
    7. Conflicts of Interest
    8. References
  26. 18 Genome Editing for Improving Abiotic Stress Tolerance in Rice
    1. 18.1 Introduction
    2. 18.2 Recent Developments in Genome Editing Technology
    3. 18.3 Challenges of Different Genome‐Editing Systems
    4. 18.4 Application of Genome‐Editing Technology for the Improvement of Abiotic Stress Tolerance in Rice
    5. 18.5 Challenges of Genome Editing in Rice
    6. 18.6 Conclusion and Future Prospects
    7. Acknowledgment
    8. Conflicts of Interest
    9. References
  27. 19 Role of Genome Engineering for the Development of Resistant Starch‐Rich, Allergen‐Free and Processing Quality Improved Cereal Crops
    1. 19.1 Introduction
    2. 19.2 Starch Characteristics
    3. 19.3 Starch Biosynthesis
    4. 19.4 Starch Digestibility and Resistant Starch
    5. 19.5 Genetic Modification in Relation to RS
    6. 19.6 Genetic Modification in Relation to Allergen‐Free Cereals
    7. 19.7 Genetic Modification in Relation to Improved Processing Quality Cereals
    8. 19.8 Conclusions
    9. Acknowledgments
    10. References
  28. 20 Engineering of Plant Metabolic Pathway for Nutritional Improvement
    1. 20.1 Introduction
    2. 20.2 Methods for Metabolic Engineering
    3. 20.3 Vitamin A
    4. 20.4 Vitamin E
    5. 20.5 Vitamin C
    6. 20.6 Vitamin B
    7. 20.7 Amino Acids and Proteins
    8. 20.8 Plant Volatiles Compound
    9. 20.9 Phytic Acid
    10. 20.10 Condensed Tannin
    11. 20.11 Conclusions
    12. Acknowledgments
    13. References
  29. 21 Genome Engineering for Food Security
    1. 21.1 Introduction
    2. 21.2 Plant Breeding for Food Security
    3. 21.3 Conclusion
    4. Conflict of Interest
    5. Acknowledgement
    6. References
  30. Index
  31. End User License Agreement

Product information

  • Title: Genome Engineering for Crop Improvement
  • Author(s): Santosh Kumar Upadhyay
  • Release date: January 2021
  • Publisher(s): Wiley
  • ISBN: 9781119672364