Genome Engineering for Crop Improvement

18 Genome Editing for Improving Abiotic Stress Tolerance in Rice

Shweta Roy^1#, Nirbhay Kumar Kushwaha^2#, Hasthi Ram³, and Praveen Soni⁴

¹ Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden

² Department of Plant Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden

³ Department of Agri‐Biotechnology, National Agri‐Food Biotechnology Institute, Mohali, Punjab, India

⁴ Department of Botany, University of Rajasthan, Jaipur, Rajasthan, India

CHAPTER MENU

• 18.1 Introduction
• 18.2 Recent Developments in Genome Editing Technology
  • 18.2.1 Meganucleases
  • 18.2.2 Zinc‐Finger Nucleases
  • 18.2.3 Transcription Activator‐like Effectors Nucleases
  • 18.2.4 Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR Associated Proteins System
• 18.3 Challenges of Different Genome‐Editing Systems
• 18.4 Application of Genome‐Editing Technology for the Improvement of Abiotic Stress Tolerance in Rice
  • 18.4.1 Salinity Tolerance
  • 18.4.2 Drought Tolerance
  • 18.4.3 Cold Tolerance
  • 18.4.4 Herbicide Tolerance
  • 18.4.5 Heavy Metal Tolerance
  • 18.4.6 Alkaline Stress Tolerance
• 18.5 Challenges of Genome Editing in Rice
  • 18.5.1 Efficient Delivery System for Genome Editing Components
  • 18.5.2 Target Specificity of the Genome‐Editing Tools
  • 18.5.3 Better Understanding of the Complex Genetic Network of Agronomic Traits
  • 18.5.4 Simultaneous Editing of Multiple Genes for Multiple Trait Improvement
  • 18.5.5 Government Policies for Genome‐Edited Crops
• 18.6 Conclusion and Future Prospects ...