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Image Processing with ImageJ - Second Edition by Javier Pascau, José María Mateos Pérez, Jurjen Broeke

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Chapter 3. Advanced Image Processing with ImageJ

The previous chapter showed you how to load and view images in ImageJ and how to make basic alterations to image intensity and pixel values. This chapter will deal with the techniques used to preprocess images. We will prepare them for image analysis and measurements. This chapter will apply some of the techniques we examined in the earlier chapters. We will cover the following topics:

  • Correcting images
  • Z-stack processing
  • Time series processing
  • Image and stack calculations

Correcting images

In order to analyze images, we sometimes need to correct the problems that were present during acquisition. Problems such as noise, uneven illumination, and background fluorescence can cause many issues during image ...

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