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Image Processing with ImageJ - Second Edition by Javier Pascau, José María Mateos Pérez, Jurjen Broeke

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Chapter 5. Basic Measurements with ImageJ

We saw in the previous chapter how to perform some preprocessing steps that prepared the image data for measurement and analysis. In this chapter, we will take a closer look at the measurement system available in ImageJ. You will also learn how to create some visualizations of movement and dynamics. We will look at the following topics in this chapter:

  • Selections and regions
  • The ROI Manager
  • Kymographs and line profiles
  • Area and line selections
  • Semiquantitative colocalization
  • Particle analysis

Selections and regions in ImageJ

We will first look at the tools that are available in ImageJ to select Regions Of Interest (ROIs). These tools can be useful if you wish to only process a small portion of your image. ROIs ...

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