September 2018
Intermediate to advanced
1178 pages
48h 30m
English
In order to detect, quantify, and study the target, it has to be purified and prepared in a suitable state for detection. This state has to assure specific detection of the target, minimizing the interference from other molecules, and noise from the environment or the detection system.
Several detection mechanisms operate on purified and concentrated solutions containing the target. Another widely diffused technique to obtain a specific response to the detection stimulus is to fix the target to a suitably prepared surface, either via an intermediate molecule, as in the ELISA assay (see Section 3.7.5) or directly, like in the technique of spin column-based nucleic acid extraction (see Section ...