
58-1
58.1 Introduction
During the early development of modern analytical chemistry, the study of natural materials was a
pr
imary
co
ncern
of or
ganic
ch
emists
an
d
bi
ologists
. A ma
jor
pr
oblem
fa
cing
th
ese
sc
ientists
wa
s
th
e
fo
r-
mulation
of me
thods
to se
parate
an
d
an
alyze
th
e
co
mplex
mi
xtures
en
countered
in bi
ological
re
search
.
Chromatography (literally “color writing”) is a physical or physicochemical technique for separation
of mi
xtures
in
to
th
eir
co
mponents
on th
e
ba
sis
of th
eir
mo
lecular
di
stribution
be
tween
tw
o
im
miscible
ph
ases
. On
e
ph
ase
is st
ationary
an
d
is in a n
ely
di
vided
st
ate
to pr
ovide
a la
rge
su
rface
ar
ea
re
lative
to
vo
lume
. e se ...