book

Micro- and Nanoengineering of the Cell Surface

by Jeffrey M Karp, Weian Zhao

May 2014

Intermediate to advanced

400 pages

17h 56m

English

William Andrew

Read now

Unlock full access

1.1 Introduction1.2 Cell Membrane Biology—Early Milestones1.3 Membrane Microdomains1.4 Cell Membrane Emergence1.5 Juxtacrine Signaling and Rewiring Cellular Networks1.6 Protein Painting, Artificial Veto Cell Engineering1.7 Trans Signal Conversion1.8 Redirecting Juxtacrine Signals1.9 Creating Auto-Signaling Loops1.10 SCP Therapeutic Flexibility1.11 The Cell Membrane FrontierReferences
2.1 Introduction2.2 Methods and Technology of Covalent Cell Surface Reaction2.3 Relevance of Covalent Cell Surface Modification2.4 Future Perspectives2.5 ConclusionsReferences
3.1 Introduction3.2 Bioorthogonal Chemical Ligation Reactions for Glycan Labeling3.3 Bioorthogonal Ligation Reactions: Exploitation in MOE-Based Applications3.4 Concluding CommentsAcknowledgmentsReferences
4.1 Bacterial Surface Display4.2 Strategies for Re-Engineering Bacterial Surfaces with Heterologous Proteins4.3 Applications of Bacteria Expressing Heterologous Surface Proteins4.4 Strategies for Re-Engineering Bacterial Surfaces with Heterologous Sugars4.5 Applications of Bacteria Expressing Heterologous Surface Sugars4.6 ConclusionAcknowledgmentReferences
5.1 Introduction5.2 Methods of Cell Surface Engineering—Applications and Recent Developments5.3 Electrostatic Interactions Mediated Cell Surface Modification5.4 Advantages and Limitations of the Noncovalent Modification of the Cell Surface5.5 Conclusions and Future PerspectivesAcknowledgmentsReferences

6.1 Introduction6.2 One-Step Protein Transfer6.3 Two-Step Protein Transfer6.4 SummaryAcknowledgmentsReferences
7.1 Introduction: Rationale for Engineering the Cell Surface7.2 Biotin and Avidin: An Overview7.3 Methods for Engineering Cell Surfaces with Avidin–Biotin Complexes7.4 Applications of Cell Surface Engineering Using Avidin–Biotin Chemistry7.5 ConclusionReferences
8.1 Computation of Cell Identity8.2 Targeted Transport of a Payload to a Cell Surface and Activation of Innate Cellular Response8.3 DNA Channels and Pores Spanning Lipid Membrane8.4 Advances in Microscopy8.5 ConclusionReferences
9.1 Introduction9.2 Use of Enzymes to Modify Cell Surface Carbohydrates on Proteins and Lipids to Enhance Migration to Tissues9.3 Modification of Enzyme Activity Leads to Changes on Cell Surface Structures that Deter Migration and Metastasis9.4 Use of Enzymes to Improve the Therapeutic Function of Cells Through Modification of the Cell Surface9.5 ConclusionReferences
10.1 Introduction10.2 Encapsulation Requirements and Strategies10.3 Materials Used for Cell Encapsulation10.4 Therapeutic Applications of Encapsulated Cells10.5 Challenges and Future PerspectivesAcknowledgmentsReferences
11.1 Introduction11.2 Imaging Modalities for NP Engineered Cells11.3 Strategies for Cell Engineering with NPs11.4 Challenges and Outlook11.5 ConclusionAcknowledgmentsReferences
12.1 Cell Therapy—Success and Current Challenges12.2 Cell Surface Engineering to Improve Cell Targeting12.3 Cell-Based Drug Delivery12.4 Concluding RemarksReferences
13.1 Introduction13.2 General Design Principles and Considerations13.3 Cell Surface Engineering with Polymer Thin Films13.4 Biomedical Applications13.5 ConclusionReferences
14.1 The 3D Extracellular Milieu14.2 Mimicking the ECM14.3 Engineering Degradability into Hydrogels14.4 Hydrogel Nanoparticles14.5 ConclusionList of AbbreviationsReferences
15.1 Introduction15.2 In Vitro Study of Cell–Cell Interactions Using Engineered Microdevices15.3 Probing and Manipulation of Cell–Cell Interactions Using Engineered Molecular Tools15.4 Conclusions and PerspectivesReferences

Content preview from Micro- and Nanoengineering of the Cell Surface

Chapter 3

Bioconjugation Reactions in Living Cells

Development, Advances, and Applications of Glycan-Specific Technologies

Laura Alberch and Kevin J. Yarema, Department of Biomedical Engineering and the Translational Tissue Engineering Center, The Johns Hopkins University, Baltimore, MD, USA

Bioorthogonal ligation reactions, in which synthetic organic chemistry can be accomplished in a physiological milieu including in living cells and animals, have flourished over the past two decades or so with the development of a profusion of coupling partners. This chapter gives an overview of bioorthogonal ligations including the best known example of this approach—the azide–alkyne “click” reaction—but also covers lesser known, more specialized, and ...