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Microarray Analysis by Kal Sharma

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CHAPTER 5

Next-Generation Sequencing Methods

Chapter Objectives

        •   Blotting

        •   Sanger Sequencing

        •   Sequencing-Based Synthesis

        •   Sequencing by Ligation

        •   Pyrosequencing

        •   DNA Sequencing through Nanopore

        •   Single-Molecule Sequencing

        •   Optical and Fluorescent Detection

The different methods for obtaining the sequence distribution of DNA are discussed here. The principle of operation, the advantages and demerits of the method, and the current and potential applications are discussed for each and every methodology. New software tools have been developed.1 The field is rapidly evolving and a glance is given here. As discussed earlier, Sanger won two Nobel Prizes, one for ...

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