The equally tempered tuning that is forced upon us by instrument manufacturers is not actually true to pure intervals. Compare, for example, a pure major third with an equally tempered major third. The tempered third is equivalent to 400 cents, while the pure major third is 384 cents—a difference of some 16 cents (or 16 percent). Through use of fine-tuning options, such as those provided by Cubase, you can now employ pure intervals. This is called just intonation.