
440 Nanosensors: Physical, Chemical, and Biological
The FeCo nanoparticles have an oxidation layer 1.5 nm thick. FeCo
nanoparticles were rst modied with amino groups on the surface by
using APTES; this resulted in approximately 660 copies of APTES molecules
on each nanoparticle. APTES-modied nanoparticles were subjected to
streptavidin-AF (Alexa Fluor) 488 modication using 1-ethyl-3-(3-dimeth-
ylaminopropyl)carbodiimide (EDC), C
8
H
17
N
3
coupling chemistry. EDC is
a water-soluble carbodiimide usually obtained as the hydrochloride and
generally employed as a carboxyl activating agent for the coupling of ...