
383Optical Nanosensors
of Rev; arginine is an amino acid, obtained from the hydrolysis or digestion
of plant and animal protein.
This reduced the QD uorescence and increased Cy5 uorescence. QD
uorescence was recovered by titrating unlabeled Rev peptide into the FRET
complex. The K
d
of the Rev–RRE complex was estimated by monitoring
the uorescence change of QD and Cy5 in this titration process. Identical
results were obtained from the titration curves from the two uorophores
(loss in FRET and increase in non-FRET emission) giving K
d
∼ 11–14 nM. This
afforded a simple method for monitoring Rev–RRE interaction and possibly
any ...