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Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences

Book Description

Practical Digital Photomicrography is an introduction to digital photography through the microscope in which readers will learn about the techniques and equipment needed to obtain high-quality images of biological subjects. Author Dr. Brian Matsumoto received his Ph.D. in Anatomy and Cell Biology from the University of California at Los Angeles in 1981. He served as the Director of the Integrated Microscopy Facility in the Department of Molecular, Cellular, and Developmental Biology at the University of California, Santa Barbara from 1992 through 2009, where he taught a graduate level course in digital microscopy.

Leveraging his decades of experience, Brian demystifies photomicrography basics such as microscope and camera concepts, illumination techniques, specimen preparation, photographic techniques, advanced optical techniques, and image post-processing. Abundant illustrations will guide the reader through these topics. Professionals in the life sciences and photography, educators and students, as well as photography enthusiasts will find this book an invaluable guide to learning the skills needed to obtain professional quality images through the microscope.

Table of Contents

  1. About the Author
  2. Copyright
  3. Acknowledgments
  4. Introduction to the Microscope
    1. Basic Microscope Parts
    2. Köhler Illumination
    3. Troubleshooting
  5. The Lens
    1. Objective Lens
    2. Viewing the Specimen
    3. Oil Immersion Objectives
    4. Depth of Focus
    5. Objective Lens: Types and Controls
    6. Water Immersion Lenses Versus Oil Immersion Lenses
    7. Role of Condenser and Field Diaphragms
    8. Focusing: Coarse and Fine
    9. Stage
    10. Substage
    11. Head of the Microscope
  6. Selecting Microscope Accessories
    1. Overview
    2. Stereomicroscopes
    3. Objectives' Limit of Resolution
    4. 1000x NA Rule
    5. Magnification Changer and Eyepiece
    6. Adding to Standard Objective Lenses
    7. Working with Live Samples in Water
    8. Condenser
    9. Stage
  7. Digital Concepts
    1. The Pixel
    2. Light Intensity and Bit Level
    3. Color Images and Additive Color
    4. Introduction to Cameras
    5. Consumer-Grade (Point-and-Shoot) Cameras
    6. Digital SLRs
    7. Dedicated Cameras
  8. Digital SLRs
    1. Introduction
    2. Indirect Mount
    3. Direct Mount
    4. Simple Tube Adapters
    5. Disadvantages of Simple Tube Adapters
    6. Advanced Adapters
    7. Avoiding Vibration on Digital SLRs
    8. Achieving Accurate Focus and Framing
    9. Olympus E3
    10. Nikon D700
    11. Nikon D300
    12. Panasonic G1
    13. Panasonic GH1
  9. Advantages of a Dedicated Digital Camera
    1. Definition
    2. Superior Imaging by Dedicated Cameras
    3. Greater Control through Binning and Gain
    4. Sensor Array and Magnification
    5. Pixel (Photosite) Size and Resolution
    6. Mounting Systems
  10. Using the Cameras
    1. Illuminating the Specimen
    2. Color Balancing the Specimen
    3. Color and Monochrome
    4. White Balance and Fluorescence Slides
    5. Optimum Digital Exposure
    6. Setting the Exposure
    7. Automatic Exposure
    8. Manual Exposure
  11. Improving the Image
    1. Introduction
    2. Coverglass Thickness and Contrast
    3. Stained Samples
    4. Unstained Samples
    5. Special Illumination Techniques
    6. Dedicated Darkfield Condensers
    7. Dedicated Dry Darkfield Condenser
    8. Dedicated Oil Darkfield Condenser
    9. Fluorescence Microscopy
    10. Difficulties of Photographing Fluorescent Specimens
    11. Color Imaging in Fluorescence
    12. Contrast by Phase Differences
    13. The Phase Contrast Image
    14. Photographing Phase and DIC Specimens
  12. Improving the Image with Software
    1. Introduction
    2. File Formats
    3. Improving the Image
    4. Enhancing Contrast in the Micrograph
    5. Color and Fluorescence Microscopy
    6. Extending the Depth of Field
    7. Extending the Dynamic Range of the Camera
  13. Buying a Microscope
    1. Used Microscopes
    2. What to Look for in a Microscope
    3. Stage
    4. Substage and Illuminator
  14. Glossary
  15. References