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Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences by Brian Matsumoto

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3.6. Adding to Standard Objective Lenses

When using a digital camera with a microscope, the goal is to determine a magnification that enlarges the specimen so it fills the frame of the camera. It is not a good idea to blow up the digital image later and hope for increased detail. Generally, any attempt to enlarge the image during post-processing will produce a blurred, unsatisfactory result.

Most microscopes come with a generic set of objective lenses: three dry lenses at 4x, 10x, 40x, and one oil immersion lens at 100x. These lenses are satisfactory for visual work, and when used with a 10x eyepiece, they give a total magnification of 40x, 100x, 400x, and 1000x respectively. However, for photographic work, these large jumps in magnification ...

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