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Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences by Brian Matsumoto

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9.5. Color and Fluorescence Microscopy

Earlier I mentioned how a color image can be generated from three black and white images. This procedure is commonly done for fluorescent micrographs taken with three colored filter sets, or cubes. The three dyes used in this technique fluoresce blue, green, or red. Figure 9-10 was photographed with a filter set that makes its emission appear blue; figure 9-11 was photographed with a filter set that makes its emission appear green; and figure 9-12 was photographed with a filter set that makes its emission appear red. Individually, there is little point in coloring these images. The black and white rendition shows the details of the staining pattern adequately. However, if you are interested in showing the ...

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