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Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences by Brian Matsumoto

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7.3. Color and Monochrome

Operating the illuminator at a specific voltage and a single color temperature might seem unimportant when photographing in black and white (figure 7-5). However, this is not the case. The color balance of the light affects the appearance of a colored specimen. For example, when imaging a colored slide with red-toned objects, you can lighten their appearance by using a light that is tinted red. The increased percentage of red light allows more light to pass through the red subject. In contrast, if you raise the color temperature so that there is a higher proportion of blue light, the red object then absorbs more of the blue light and appears darker. When you use high voltage and intense lighting, the incident light ...

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