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Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences by Brian Matsumoto

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7.2. Color Balancing the Specimen

If the goal is to capture realistic colors in the specimen, you need to consider the contribution that the light source plays in tinting the overall shade. Visually, the human eye can report color under a variety of lighting regimes. For example, a green-colored plant has the same color whether it is viewed with indoor or outdoor lighting. However, the camera is not as versatile, and it is incapable of adjusting for the effects of altered lighting. Its recording of the color green depends on the ambient lighting conditions.

Another example is apparent when using a tungsten illuminator with a microscope. When compared to daylight, this light has a preponderance of red. Quantitatively, this color is defined in ...

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