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Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences by Brian Matsumoto

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8.2. Coverglass Thickness and Contrast

A thin glass cover, or coverglass, is used to view most material under a microscope. If the coverglass is too thick or thin, the image will be blurred and lack contrast. This has been described in chapter 2, but the subject will be repeated here with the aim of providing ways to improve the photograph.

The easiest solution is preventative: always prepare the sample so that the coverglass thickness is 0.17 mm. Many investigators do not realize that microscopes are designed to work optimally with a coverglass of this thickness. A comparison of two images shows how incorrect coverglass thickness reduces contrast and image definition (figure 8-1, figure 8-2). Coverglasses are sold in a variety of thickness ...

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