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Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences by Brian Matsumoto

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9.4. Enhancing Contrast in the Micrograph

Many micrographs suffer from a lack of contrast—a common situation because living cells and unstained specimens are transparent, with no areas that are deep black or brilliant white. This is illustrated in figure 9-4 of a diatom photographed with brightfield illumination.

When a RAW file from a digital SLR is opened, there are several controls to adjust the image and improve its appearance. Referring back to figure 9-2, you can see the controls labeled Exposure, Contrast, Brightness, Recovery, and Fill Light in the Bridge program from Adobe. Adjusting these controls allows you to brighten or darken the image, recover intensity variations in highlighted regions, and lighten dark areas to reveal shadows. ...

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