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Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences by Brian Matsumoto

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1.2. Köhler Illumination

  1. Turn on the microscope illuminator and make sure light is passing through the field diaphragm.

  2. Place a slide (a slide of stained tissue is good) on the microscope stage. Secure the slide with the microscope's clips. Make sure the coverglass is facing up.

  3. Rotate the objective marked 10x into position. It will be perpendicular to the stage. Make sure it will clear the clips that hold the slide before rotating it into place.

  4. Using the coarse focus knob, move the stage to just short of the 10x objective. Be careful not to hit the objective into the slide (see figure 1-4). You should now be able to see an out-of-focus specimen.

    Figure 1.6. When you close the field diaphragm to its minimum size, you will see a circle of light ...

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