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Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences by Brian Matsumoto

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3.5. Magnification Changer and Eyepiece

As mentioned earlier, magnification is a multiplicative function—it is determined by multiplying the power of the objective by that of the eyepiece plus any intermediate magnification device. In newer microscopes, many camera adapters have a 1x factor so that the magnification to the camera is simply that of the objective lens. However, if you have a projection eyepiece, you can insert it in the camera tube to achieve either greater or lesser magnification. There are adapters that increase power by 2x or decrease it by half (0.5x). Which of these you use depends on the NA of the objective and its power. You want the magnification to stay within the range of usefulness and not in the range of empty magnification. ...

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