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Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences by Brian Matsumoto

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2.1. Objective Lens

The objective lens projects a real image of the specimen up toward the eyepiece, and its design and quality determine image sharpness. An understanding of its optics and specifications enables you to determine the most appropriate magnification and illumination for visualizing the specimen.

The first concept we will investigate is calculating the limits of seeing fine detail with a microscope. Microscopists have an equation to determine the minimum distance that can be resolved by an objective lens. This equation has three factors that influence the ability to resolve fine detail: the angle of light captured by the objective, the medium that lies between the objective and the specimen, and the wavelength of light that illuminates ...

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