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Practical Digital Photomicrography: Photography Through the Microscope for the Life Sciences by Brian Matsumoto

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2.2. Viewing the Specimen

Although it seems counterintuitive, the image seen through the microscope is not a direct representation of the subject being observed. Light interacts with the system, and its wavelike property is responsible for image formation. Light rays can bend, interact with other light rays, and react to obstacles in their path. Light also interacts with the specimen on the microscope's stage as it passes from below, traveling through and around the specimen. The objective, eyepiece, and mounting medium, to name a few components, all play an integral part in how the image appears through the eyepiece.

The following experiment by Ernst Abbe demonstrates how light rays bend and interact with each other to form what is seen through ...

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