Scientific Imaging with Photoshop: Methods, Measurement, and Output

Chapter 3. Guidelines for Specific Types of Images

Human beard hair stained with CY3 and acquired on a BioRad 1024 confocal microscope system with LaserSharp 3.1 software (BioRad Corporation, Hercules, CA). Image was colorized in and color conformed to CMYK publication in Photoshop CS3 Extended (Adobe Systems Incorporated, San Jose, CA). Scale bar = 10 microns. Image courtesy of Marna Ericson, Ph.D.

WHILE SOME CHANGES to images are universally applicable, other changes are specific to the intent. Based on intent, the most limited number of changes (or none at all) is applied to those images intended for optical density/intensity measurement (OD/I), ...