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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
xiii
Preface
Second-harmonic generation (SHG) microscopy began to emerge as a high-resolution optical imaging
modality in the late 1990s, about 10 years aer two-photon excited uorescence (TPEF) microscopy
was pioneered by the laboratory of Watt Webb. Concurrent with two-photon microscopy, the initial
suggestion for SHG imaging was made by Colin Sheppard. Although largely unnoticed by the biomedi-
cal optics community, Isaac Freund and coworkers published a ber alignment study in rat-tail ten-
don using SHG polarization analysis, and while this was performed at low resolution (~50 μm), this
landmark study captured the essence of using SHG to ...
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Publisher Resources

ISBN: 9781439849156