
Figure 5.9 Eect of myosin extraction on SHG from myobrils. Panels a and b show myobril SHG images
before and aer myosin extraction, respectively. Panels c and d show two-photon excitation images of actin labeled
with rhodamine-phalloidin before and aer myosin extraction, respectively. Panels e and f show a superposition of
SHG image (in red) and TPE image (in green) before and aer the extraction. e size of the images is 20 × 20 μm
2
.
(Modied from Vanzi, F. et al. 2006. J Muscle Res Cell Motil 27, 469–479. With permission.)