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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
Figure 15.2 SHG/2PEF imaging of lung brosis in bleomycin-treated mice. SHG (green: collagen) and 2PEF
(red: elastin, macrophages) signals excited with 50 mW excitation power at 860 nm. Yellow color: SHG/2PEF colo-
calization. (a) Fresh unlabeled brotic lung (day 14), at 42 μm under the pleura, evidencing the heterogeneous
brosis distribution; (b) 3D reconstruction of the SHG signal from the underlined area in (a), within the tissue (b1)
and in the pleurae (b2); (c) unstained histological sections embedded in paran, from (c1) control, (c2) day 3, (c3)
day 7, and (c4) day 14 bleomycin lungs. Bleomycin treatment induces (i) inammation, whic ...
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Publisher Resources

ISBN: 9781439849156