
Figure 15.2 SHG/2PEF imaging of lung brosis in bleomycin-treated mice. SHG (green: collagen) and 2PEF
(red: elastin, macrophages) signals excited with 50 mW excitation power at 860 nm. Yellow color: SHG/2PEF colo-
calization. (a) Fresh unlabeled brotic lung (day 14), at 42 μm under the pleura, evidencing the heterogeneous
brosis distribution; (b) 3D reconstruction of the SHG signal from the underlined area in (a), within the tissue (b1)
and in the pleurae (b2); (c) unstained histological sections embedded in paran, from (c1) control, (c2) day 3, (c3)
day 7, and (c4) day 14 bleomycin lungs. Bleomycin treatment induces (i) inammation, whic ...