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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
Theoretical Framework forSHG Microscopy 13
1.3.3 Inuence of Tight Focusing on the Local Polarization: Beyond the
Paraxial Approximation
Under conditions of tight focusing, the local elds experienced within the confocal volume are gener-
ally distinct from those introduced in the far eld. e reasons for this eect are depicted qualitatively
in Figure 1.4. In brief, the high angles of incidence arising when using high NA objectives introduce
eld components in all three directions even if starting with linearly polarized light. e most com-
mon strategies developed for modeling the local elds generally consider the focusing of a Gaussian
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Publisher Resources

ISBN: 9781439849156