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Over recent years, second-harmonic generation (SHG) microscopy has been well established as a micro-
scopic imaging modality used in biophysics, biomedical optics, and biophotonics applications. It has
already been widely demonstrated that SHG is a powerful technique for both cellular and tissue imag-
ing. However, a commercial microscope exclusively dedicated to SHG imaging is still lacking. Very
oen, SHG microscopes used in laboratory research are adapted from commercial (confocal or multi-
photon) microscopes. However, for some applications, ex novo design is required to t the technical
specications of the experiments to be performed ...