
Second Harmonic Generation Imaging26
According to these equations, a beam focus can be scanned across the focal plane by tilting the laser
beam with respect to the optical axis. is is in general achieved by using a scanning system, which
allows tilting the laser beam from the optical axis (see Section 2.2.1 for a detailed description).
In a typical experimental setup (Denk et al., 1990), a telescope is inserted between the scanning
system and the focusing lens (objective lens). is telescope has several functions. First of all, it allows
re-conjugating the output of the scanning system with the input of the focusing objective lens. ...