
How to Build an SHGApparatus 31
e AOD makes use of the acoustic frequency f dependent diraction angle, where a change in the
angle Δ
ϕ
can be expressed as a function of the change in frequency Δf:
∆ ∆φ
λ
=
v
f
(2.4)
where
λ
and ν are the acoustic wavelength and velocity of the acoustic wave, respectively.
e rst implementation of a high-speed, random-access, laser-scanning uorescence microscope
congured to record fast physiological signals from small neuronal structures with high spatiotempo-
ral resolution has been presented by Bullen etal. (1997). Recently, Sacconi etal. (2008) combined the
advantages of SHG with an AOD-based random-access ...