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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
Second Harmonic Generation Imaging36
2.2.7 A Multifocal SHG Microscope
To overcome the limitations in scanning time required for imaging applications (see also Section 2.2.4),
an SHG microscope can be tted with a multifocal excitation scheme, allowing fast scanning of a
selected eld of view by multiple laser beamlets.
A multifocal SHG microscope is built in the same manner of a multifocal multiphoton microscope
(MMM) with the only dierence of detecting SHG light instead of two-photon uorescence. e dierences
reside in the detection system and in particular in the optical ltering, while the excitation system is exactly ...
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Publisher Resources

ISBN: 9781439849156