
Second Harmonic Generation Imaging36
2.2.7 A Multifocal SHG Microscope
To overcome the limitations in scanning time required for imaging applications (see also Section 2.2.4),
an SHG microscope can be tted with a multifocal excitation scheme, allowing fast scanning of a
selected eld of view by multiple laser beamlets.
A multifocal SHG microscope is built in the same manner of a multifocal multiphoton microscope
(MMM) with the only dierence of detecting SHG light instead of two-photon uorescence. e dierences
reside in the detection system and in particular in the optical ltering, while the excitation system is exactly ...