Second Harmonic Generation Imaging46
• e laser source (Ti:sapphire) providing femtosecond pulses. See Section 2.2.2 for a detailed
description of suitable laser sources for SHG microscopy.
• e power adjustment system (Section 2.2.3), made by a rotating half-wave plate (
λ
/2), (remotely
controlled from PC), and a polarizing beam-splitter cube (PBS).
• e scanning system (Section 2.2.4), made with two orthogonal galvo-mirrors (G
x
and G
y
) opti-
cally relayed by a telescope (L1,L2) with magnication equal to 1. A detailed description of the
basic principle of a scanning system is provided in Section 2.2.1.
• e telescope (L3,TL) for magnifying the beam size and de-multiply the scanned angle. See
Section 2.2.1 for a de ...