
Second Harmonic Generation Imaging66
Combined THG/SHG imaging can be used to detect a number of key events during cell divisions.
SHG reveals the formation of mitotic spindles, providing a specic marker of cell divisions. e SHG
intensity exhibits a nearly Gaussian temporal behavior with a maximum intensity corresponding to the
anaphase, permitting the measurement of division timings and cell cycle duration with high precision.
Moreover, THG, SHG, and 2PEF signals from red uorescent proteins can be produced and detected
simultaneously. Figures 3.12c through 3.12e show an example of multimodal THG−SHG−2PEF imag-
ing of H2B-mCherry mutant embryo ...