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Second Harmonic Generation Imaging
book

Second Harmonic Generation Imaging

by Francesco S. Pavone, Paul J. Campagnola
April 2016
Intermediate to advanced content levelIntermediate to advanced
476 pages
21h 10m
English
CRC Press
Content preview from Second Harmonic Generation Imaging
96 Second Harmonic Generation Imaging
the target to be in a bulk noncentrosymmetric microenvironment, THG does not have this constraint.
However, THG is not highly versatile as a change in refractive index is required when focused beams
are used. is is because the signal arises from optical heterogeneities in the specimen where there are
changes in the third-order nonlinear susceptibility,
χ
(3)
. One point of comparison comes from imag-
ing biological membranes with both SHG and THG. SHG images the two-dimensional membrane
itself where dye molecules have assembled parallel to each other [16,21,57]. In contrast, THG does so
by probing the volu ...
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Publisher Resources

ISBN: 9781439849156